Detection of flavonoids in Alpinia purpurata ( Vieill . ) K . Schum . leaves using high - performance liquid chromatography
نویسنده
چکیده
The species Alpinia purpurata is scarcely cited as to ethnopharmacology and phytochemistry. This study aimed to analyze bioactive compounds through high-performance liquid chromatography (HPLC). Hydroalcoholic crude extract was obtained from A. purpurata dried leaves. Folin-Ciocalteau method was used to quantify total phenols, using gallic acid as standard. The obtained result was 15.6 mg GAE g-1. The crude extract was partitioned with the solvents ethyl acetate and butanol, followed by thin-layer chromatography (TLC) and HPLC. The flavonoids kaempferol-3-O-glucuronide and rutin were detected at a higher concentration in ethyl acetate and butanolic extracts. The butanolic extract contains the highest flavonoid percentage (94.3%). A. purpurata presents important flavonoids of therapeutic use, already verified for A. zerumbet. This is the first study verifying the presence of flavonoids in A. purpurata extracts.
منابع مشابه
Selected Zingiberaceae Species Exhibiting Inhibitory Activity Against Mycobacterium tuberculosis H37Rv: A Phytochemical Profile
As part of the research efforts to identify plant species which may have potential against tuberculosis, a study was earlier conducted in collaboration with the institute for TB Research, university of illinois, Chicago, to randomly screen the crude alcoholic extracts of different plant species using the MABA assay, to determine any inhibitory activity against the causative agent, Mycobacterium...
متن کاملTolerance of tropical fruits and a flower to carbonyl sulfide fumigation
The tolerance of ‘Apple’ banana (Musa sp.), avocado (Persea americana Mill.), mango (Mangifera indica L.), papaya (Carica papaya L.), and red ginger (Alpinia purpurata (Vieill.) K. Schum) inflorescences to carbonyl sulfide (COS) fumigation was studied. Commodities were exposed at 25°C to COS at various concentrations (1–6% (v/v) for banana; 1% and 2% for the other fruits for various times from ...
متن کاملQuantitative analysis of flavonoids in the flowers and leaves of Ficaria verna Huds.
The quantitative determination of flavonoid compounds in flowers and leaves of Ficaria verna Huds. (Ranunculaceae) was carried out in different growing seasons of the plant, using Christ-Müller's method (Polish Pharmacopoeia, 1999) and high performance liquid chromatography (HPLC) analysis after acid hydrolysis. The flavonoid content was much higher in flowers than in leaves.
متن کاملDetermination of some B Vitamins in Sour Cherry Juice Using Dispersive Liquid-liquid Microextraction Followed by High-performance Liquid Chromatography
Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile ph...
متن کاملUse of Temperature-controlled Ionic Liquid-assisted Dispersive Liquid-Liquid Microextraction Method for the Detection of Amoxicillin, Cloxacillin and Erythromycin Residues in Cow Milk using High Performance Liquid Chromatography
Background and Objectives: Different types of antibiotics are used indiscriminately in animal husbandry for the growth and treatment of livestock, which cause accumulation of antibiotic residues in livestock products such as milk. Long-term consumption of milk contaminated with antibiotic residues above the permissible limits is harmful to human health, causing antibiotic resistance problems. T...
متن کامل